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A detailed description of gene expression in all macroanatomical brain regions and in species with similar morphologies and behaviors is however lacking. Here, we dissected the brain of two cichlid species into six macroanatomical regions. Ophthalmotilapia nasuta and O. Our results show that gene expression differs substantially between all six brain parts within species: out of 11, assessed genes, 8, are differentially expressed DE in at least one brain part compared to the average expression of the other brain parts. Functional differences between brain parts were consistent between species. Only 32 genes show significant differences in fold change across brain parts between species. These genes are mainly linked to transport, transmembrane transport, transcription and its regulation and signal transduction.

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