Get this from a library! Prescott & Dunn's industrial microbiology.. [Samuel Cate Prescott; Cecil Gordon Dunn; Gerald Reed]. Book Source: Digital Library of India Item aracer.mobi: Prescott, Samuel aracer.mobiioned. Prescott and Dunn's Industrial Microbiology. Front Cover. Gerald Reed. CBS Publisher, - Industriele mikrobiologie - pages. 0 Reviews.
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Outline of Microbial Taxonomy Metabolism. 3. Yeasts M W Miller. 15 Bibliographic information. QR code for Prescott & Dunn's industrial microbiology . Prescott & Dunn's Industrial Microbiology. Front Cover. Gerald Reed. CBS Publishers & Distributors PVT. Limited, - Industrial microbiology - pages. Prescott & Dunn's Industrial Microbiology book. Read 3 reviews from the world's largest community for readers.
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Without inverting, the plate is incubated at a suitable temperature. Any growth observed in the lower layer can now be regarded as growth stimulated by lysine diffused from the colony just above in the first agar.
The corresponding colony in the agar surface 1st plate can now be subcultured for further assay. The method is normally called Crowded Plate method. Take 10 g soil and prepare dilution 2. Transfer about 2 loopfuls of sample suspension on the agar plate labeled '1' 3.
Spread the suspension over the entire surface with a dally rod 4. Use the residue of the dally rod as an inoculum for the plate labeled '2' 5.
Spread thoroughly as in step 3 Figure 2. Carry out the sequential transfers to 3 other properly labeled plates. Use the methods given in steps 1 to 6 7. Incubate inverted all the plates at 25C for days.
Observe for the zones of inhibition in between 8. Subculture the selected colonies from the crowded plate 9. An overview of the screening of industrially important microorganisms is given in figure 2. Several selection techniques are available at present to isolate microorganisms of our interest from any environment. Basically, such methods function by facilitating the growth of the desired species so that the subsequent isolation becomes easier.
There are three main groups of selection methods: Chemical Physical Biological The basic strategy in all of the above methods is to create environment conducive to physical segregation or even encouragement of the growth of the desired species while discouraging or even inhibiting the rest.
Figure 2. Incubation temperature: selection of psychrophiles, thermophiles, etc. Cell size and motility: microorganisms can be selected based on size by using filters of varying pore sizes.
Sometimes, animals can serve as a reservoir of a given species of microorganism. For example, when sputum organisms from a patient suffering from streptococcal pneumonia are injected into laboratory mice, all organisms but Streptococcus pneumoniae are killed by the defense mechanism of the mouse. The mouse thus becomes, in a sense, a biological reservoir of pneumococci! It now becomes easier to carry out further isolation works. The descendant of a single isolation in pure culture constitutes a strain.
A strain is usually made up of succession of cultures and is often derived from a single colony. If a strain is derived from a single parental cell, it is termed a clone. Pour-plate technique Spread-plate technique Figure 3. In it, the sample broth is streaked onto a dry agar surface in a series of non-overlapping streaks see figure 3.
The process thins out the cells and at some point the cells are separated sufficiently apart to give rise to discrete colonies.
The method has some limitations in that psychrophiles or organisms that cannot withstand a temporary shock of C cannot survive. Besides, the isolated colonies remain embedded in the medium and subculturing them entails digging through the agar. The single most important advantage in it is, it can be used for quantitative enumeration purpose also. A variation of this method is to transfer 0.
The plate is rotated gently in the shape of 8 to affect uniform mixing. Turntables can be used for the spreading. In manual method, a loopful of culture is transferred to the agar surface and spread uniformly with a bent glass rod called dally rod. To affect finer isolation, the residue in the rod is used to spread-plate yet another fresh plate see figure 3. In this way, plates can be used for the spreading. As the spreading progresses, at some point the cells will be sufficiently apart to the affect isolation.
A small segment of mycelia that radiate outward can be aseptically cut out and place on a fresh medium for growth. With this method, an unequivocal selection of a single cell is possible.
The method uses an instrument called micromanipulator a high resolution microscope fitted with manipulating ancillary and needs considerable expertise.
During manipulation, the cell is first identified. Isolation takes place on an agar surface. A sharp needle provided with the manipulator is brought close to the cell.
The needle is allowed to rest on the agar surface near the cell so that a small dent is formed. Next, the field is shifted away from the needle tip. The dented impression made by the needle during the shifting causes the cell to follow the course of the needle and thus gets separated.
The cell is later cut out from the agar surface and subcultured see figure 3. Figure 3. As a result of this modification, progressive improvement in the yield can be anticipated.
Gyles Editor , John F. Prescott Editor , J. Glenn Songer Editor , Charles O. Thoen Editor. AVI Pub.
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