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Seeds were sterilized and inoculated in Murashige and Skoog media supplemented with vitamins, myo-inositol, sucrose, and agar. Gas exchange was evaluated in the germination stage by comparing 3 flask-sealing systems: rigid polypropylene lids PLs without vents, PLs with 1 vent, and PLs with 2 vents covered with membranes. In the regeneration stage, cotyledon and hypocotyl segments were transferred to the organogenesis-inducing media, being the different sealing types also tested in a factorial scheme. Photosynthetic pigments, morphological and hystological analyses were conducted for each treatment. Plants maintained in glass flasks capped with vented lids showed more vigorous growth and differentiated anatomical structures. These treatments resulted in taller plants, higher numbers and more expanded leaves, higher fresh and dry weights, and an increase in photosynthetic pigments. Cultivation of C. During the regeneration stage, hypocotyls were more effective than cotyledons. Sealing type influenced the morphogenic responses of pepper, demonstrating that an increase in gas exchange has a positive effect on biomass production and acclimatization of the plantlets. O cultivo de C. However, this process is complicated due differences in variables, such as relative humidity RH , temperature, ventilation, and nutrient concentrations between the ex vitro environment and the in vitro environment CHA-UM et al. Lids that allow higher gas exchange through membranes were used to improve ventilation inside culture flasks, reducing RH and thereby increasing transpiration and effective water and nutrient absorption in plants KOZAI, ; XIAO et al. MilliSeal r membranes have been successfully used to improve gas exchange in the culture flask in the propagation of many species, including Pfaffia glomerata IAREMA et al. Unlike other members of the Solanaceae family, which have morphogenesis easily reproduced protocols, chillies and peppers of the genus Capsicum are recalcitrant to in vitro culturing.

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